ELISA試驗(yàn)“溫育”操作是不是只能在37℃條件下完結(jié)

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育是</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">測定中影響測定勝敗最為關(guān)鍵的一個(gè)要素。</font><font face="Calibri">ELISA</font><font face="宋體">實(shí)驗(yàn)作為一種固相免疫測定，抗原抗體的聯(lián)絡(luò)反應(yīng)在固相上進(jìn)行，要使液相中的抗原或抗體與固相上的特異抗體或抗原徹底聯(lián)絡(luò)，必須在一定的溫度條件下反應(yīng)一定的時(shí)間。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育所需時(shí)間與溫度成反比，即溫度越高，則所需時(shí)間相對(duì)較短。在</font>ELISA<font face="宋體">中一般有兩次抗原抗體反應(yīng)，即加標(biāo)本和加酶聯(lián)絡(luò)物后。抗原抗體反應(yīng)的完結(jié)需要有一定的溫度和時(shí)間，這一保溫進(jìn)程稱為溫育</font><font face="Calibri">(incubation)</font><font face="宋體">，也被稱之為孵育。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">這是一個(gè)逐漸平衡的進(jìn)程，因而需經(jīng)渙散才華到達(dá)反應(yīng)的結(jié)尾。在這今后參加的酶符號(hào)抗體與固相抗原的聯(lián)絡(luò)也相同如此。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育常選用的溫度有</font><font face="Calibri">43</font><font face="宋體">℃、</font><font face="Calibri">37</font><font face="宋體">℃、室溫文</font><font face="Calibri">4</font><font face="宋體">℃（冰箱溫度）等。</font><font face="Calibri">37</font><font face="宋體">℃是實(shí)驗(yàn)室中常用的保溫溫度，也是大多數(shù)抗原抗體聯(lián)絡(luò)的適宜溫度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">實(shí)驗(yàn)標(biāo)明，兩次抗原抗體反應(yīng)一般在</font>37<font face="宋體">℃經(jīng)</font><font face="Calibri">1-2</font><font face="宋體">小時(shí)，產(chǎn)物的生成可達(dá)高峰。為加快反應(yīng)，可前進(jìn)反應(yīng)的溫度，</font><strong><font face="Calibri">ELISA</font><font face="宋體">試劑盒</font></strong><font face="宋體">有些實(shí)驗(yàn)在</font><font face="Calibri">43</font><font face="宋體">℃進(jìn)行，但不宜選用更高的溫度?？乖贵w反應(yīng)</font><font face="Calibri">4</font><font face="宋體">℃更為徹底，在放射免疫測定中多使反應(yīng)在冰箱中放置一晚上，以構(gòu)成最多的堆積。但因所需時(shí)間太長，在</font><font face="Calibri">ELISA</font><font face="宋體">中一般不予選用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>