ELISA試劑盒中幾大要點(diǎn)影響非特異性顯色的

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">影響</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">中非特異性顯色的緣由許多，如試劑盒特異性、查驗標(biāo)本中含酶符號物的煩擾物，操作進(jìn)程中的疑問。這篇文章就這一緣由作一簡明剖析</font><font face="Calibri">,</font><font face="宋體">以便能夠經(jīng)過以上辦法把非特異顯色降至最低極限，然后行進(jìn)檢測的特異性，并得到更精確、牢靠的試驗作用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">查驗標(biāo)本中富含酶符號物的煩擾物</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">內(nèi)源性煩擾物：類風(fēng)濕因子</font>(RF)<font face="宋體">、黃疸等，類風(fēng)濕因子是可作用于多種動物以及人</font><font face="Calibri">IgGFc</font><font face="宋體">段的本身抗體，大都為</font><font face="Calibri">IgM</font><font face="宋體">類，能充任抗原成分與固相及酶標(biāo)抗體反響，然后出現(xiàn)非特異性顯色。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">黃疸血標(biāo)本中常富含內(nèi)源性過氧化物酶，如用辣根過氧化物酶為符號物，就有能夠發(fā)作非特異性顯色。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">外源性煩擾物，常常因樣品收集、儲存、處置不妥構(gòu)成如樣品溶血，被細(xì)菌污染，標(biāo)本凝聚不全等。溶血標(biāo)本，紅細(xì)胞溶解割裂，釋放出血紅素構(gòu)成，而血紅素中的鐵卟啉是過氧化物酶的類似物，以</font>HRP<font face="宋體">為符號的</font><font face="Calibri">ELISA</font><font face="宋體">測定中，溶血標(biāo)本能夠會添加非特異性顯色。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">如有細(xì)菌污染，菌體中能夠富含內(nèi)源性</font>HRP<font face="宋體">，有國內(nèi)報導(dǎo)酶免</font><font face="Calibri">HAg</font><font face="宋體">試劑檢測溶血標(biāo)本時可構(gòu)成假陽性。如在冰箱中保管過久，其間的</font><font face="Calibri">IgG</font><font face="宋體">可發(fā)作聚合，在直接法</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒中可使本底加深。因而，血標(biāo)本在收集處置時應(yīng)留心，在冰箱中保管不易過久。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>